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pierceable foil seal  (Bio-Rad)


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    Structured Review

    Bio-Rad pierceable foil seal
    Pierceable Foil Seal, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1091 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcr+plate+heat+seal/PCR+Plate+Heat+Seal/pm42107190-88-20-23
    Average 96 stars, based on 1091 article reviews
    pierceable foil seal - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Generated:

    Article Title: DNA-PKcs inhibitor AZD7648 reveals sgRNA cross-contaminants and enhanced sensitivity of genome engineering off-target activity in HSPCs
    Article Snippet: .. Droplets were generated using Automated Droplet Generation Oil for Probes (Bio-Rad) and DG32TM Automated Droplet Generator Cartridges with the automatic droplet generator (Bio-Rad AutoDG), and the plate sealed with a PCR Plate Heat Seal (Bio-Rad) and PX1TM PCR Plate Sealer (Bio-Rad). .. PCR was performed using the C1000 TouchTM Thermal Cycler with 96-Deep Well Reaction (Bio-Rad) and droplets read using a QX600 Droplet Reader (Bio-Rad).

    Article Title: Unveiling the cut-and-repair cycle of designer nucleases in human stem and T cells via CLEAR-time dPCR
    Article Snippet: .. Droplets were generated using Automated Droplet Generation Oil for Probes (Bio-Rad) and DG32TM Automated Droplet Generator Cartridges with the automatic droplet generator (Bio-Rad QX200 AutoDG), and the plate was sealed with a PCR Plate Heat Seal (Bio-Rad) and PX1TM PCR Plate Sealer (Bio-Rad). .. PCR was performed using the C1000 TouchTM Thermal Cycler with 96-Deep Well Reaction (Bio-Rad) and droplets read using a QX200 Droplet Reader (Bio-Rad).

    Article Title: DNA-PKcs inhibitor AZD7648 reveals sgRNA cross-contaminants and enhanced sensitivity of genome engineering off-target activity in HSPCs.
    Article Snippet: .. Droplets were generated using Automated Droplet Generation Oil for Probes (BioRad) and DG32TM Automated Droplet Generator Cartridges with the automatic droplet generator (Bio-Rad AutoDG), and the plate sealed with a PCR Plate Heat Seal (Bio-Rad) and PX1TM PCR Plate Sealer (Bio-Rad). .. PCR was performed using the C1000 TouchTM Thermal Cycler with 96-Deep Well Reaction (Bio-Rad) and droplets read using a QX600 Droplet Reader (Bio-Rad).

    Article Title: Unveiling the cut-and-repair cycle of designer nucleases in human stem and T cells via CLEAR-time dPCR.
    Article Snippet: .. Droplets were generated using Automated Droplet Generation Oil for Probes (Bio-Rad) and DG32TM Automated Droplet Generator Cartridges with the automatic droplet generator (Bio-Rad QX200 AutoDG), and the plate was sealed with a PCR Plate Heat Seal (Bio-Rad) and PX1TM PCR Plate Sealer (Bio-Rad). .. PCR was performed using the C1000 TouchTM Thermal Cycler with 96- Deep Well Reaction (Bio-Rad) and droplets read using a QX200 Droplet Reader (Bio-Rad).

    Polymerase Chain Reaction:

    Article Title: DNA-PKcs inhibitor AZD7648 reveals sgRNA cross-contaminants and enhanced sensitivity of genome engineering off-target activity in HSPCs
    Article Snippet: .. Droplets were generated using Automated Droplet Generation Oil for Probes (Bio-Rad) and DG32TM Automated Droplet Generator Cartridges with the automatic droplet generator (Bio-Rad AutoDG), and the plate sealed with a PCR Plate Heat Seal (Bio-Rad) and PX1TM PCR Plate Sealer (Bio-Rad). .. PCR was performed using the C1000 TouchTM Thermal Cycler with 96-Deep Well Reaction (Bio-Rad) and droplets read using a QX600 Droplet Reader (Bio-Rad).

    Article Title: Preclinical Development of T Cells Engineered to Express a T-Cell Antigen Coupler Targeting Claudin 18.2–Positive Solid Tumors
    Article Snippet: Both gDNA and cDNA were used in the QX200 instrument (Bio-Rad Laboratories) at 100 ng/reaction in a droplet digital PCR assay (ddPCR). ddPCR was performed following standard procedures as per Bio-Rad Droplet Digital PCR Application Guide (Bulletin 6407). .. The reagents used were nuclease-free water (IDT Technologies, 11-05-01-04), ddPCR supermix for probes (no dUTP), 500 reactions (Bio-Rad, 1863024), DG8 cartridges for the QX100/QX 200 Droplet generator (Bio-Rad, 1864008), Droplet generation oil for probes (Bio-Rad, 1863005), ddPCR 96-well semi-skirted plates (Bio-Rad, 12001925), Droplet generator DG8 gasket (Bio-Rad, 1863009), PCR plate heat seal, and foil, pierceable (Bio-Rad, 1814040). ..

    Article Title: Proximity sequencing for the detection of mRNA, extracellular proteins and extracellular protein complexes in single cells
    Article Snippet: 5× Maxima H minus RT buffer (Thermo Scientific, cat. no. EP0752) Ficoll Solution (20%; Sigma-Aldrich, cat. no. F5415–25 ml) Exonuclease I and 1× exonuclease I buffer ((New England Biolabs, cat. no. M0293) Nextera XT DNA library preparation kit (Illumina, cat. no. FC-131–1024) Chromium Next GEM Chip G single cell kit (10× Genomics, cat. no. 1000127) Chromium Next GEM single cell 3′ GEM, library and gel bead kit v.3.1 (10× Genomics, cat. no. 1000268) Dual index kit TT set A, 96 reactions (10× Genomics, cat. no. 1000215) AMPure XP (Beckman Coulter, cat. no. A63881) Buffer EB (Qiagen, cat. no. 19086) Agilent high-sensitivity D5000 ScreenTape (Agilent, cat. no. 5067–5592) Agilent high-sensitivity D5000 reagents (Agilent, cat. no. 5067–5593) Agilent HS NGS fragment kit (1–6,000 bp), 500 (Agilent, cat. no. DNF-474–0500) Qubit 1× double-stranded DNA high-sensitivity assay kit (Invitrogen, cat. no. Q33231 ) Qubit assay tubes (Invitrogen, cat. no. Q32856 ) KAPA HiFi HotStart ReadyMix (2×; KAPA Biosystems, cat. no. KK2601) SPRIselect beads (Beckman Coulter, cat. no. B23317 ) NextSeq PhiX control kit (Illumina, cat. no. FC-110–3002) NextSeq 500/550 mid-output kit v2.5 (150 cycles; Illumina, cat. no. 20024904) NextSeq 500/550 high-output kit (150 cycles; Illumina, cat. no. 20024907) 10% Sarkosyl Solution (1,000 ml; Teknova, cat. no. S3377) dl -Dithiothreitol solution (DTT, 1 M; Sigma-Aldrich, cat. no. 43816–10 ml) .. Microcentrifuge tube, 1.5 ml DNA LoBind tube, 1.5 ml (Eppendorf, cat. no. 022431021) Falcon polystyrene conical tube, 15 and 50 ml Filter tips (P1000, P200, P100 and P10) Rainin tips (P20 and P200) 96-well plate (Eppendorf, Bio-Rad) Hemocytometer (Hausser Scientific, cat. no. 3520) Falcon cell strainers, 40 μm (Fisher Scientific, cat. no. 08–771-1) Magnetic disc (V&P Scientific, cat. no. 772DP-N42–5-2) Plastic tubes (Scientific Commodities, cat. no. BB31695PE/2) Magnetic stirrer (V&P Scientific, cat. no. 710D2) DiaMag 0.2-ml magnetic rack (Diagenode, cat. no. B04000001) DynaMag-96 side magnet (Invitrogen, cat. no. 12331D) Themal cycler (Bio-Rad C1000 Touch, cat. no. 1851196) PX1 PCR plate sealer (Bio-Rad, cat. no. 1814000) PCR plate heat seal, foil, pierceable (Bio-Rad, cat. no. 1814040) Adhesive PCR plate foils (Thermo Scientific, cat. no. AB-0626) Nanodrop UV–visible spectrophotometer (Thermo Scientific, cat. no. ND-ONE-W) Qubit 4.0 fluorometer (Invitrogen, cat. no. Q33238 ) Agilent 5200 fragment analyzer (Agilent) Agilent 4200 TapeStation (Agilent) Fortessa 4–15 (BD Biosciences) Cell sorter (BD FACSAria II) Pump 11 Pico Plus Elite infusion/withdrawal programmable dual syringe pump (Harvard Apparatus, cat. no. 70–4506) Chromium Controller (10× Genomics, cat. no. 1000204) NextSeq 500/550 (Illumina) .. TE-TW (10 mM Tris pH 8.0, 1 mM EDTA, 0.01% (wt/vol) Tween-20 and nuclease-free water) TE-SDS (10 mM Tris pH 8.0, 1 mM EDTA, 0.5% (wt/vol) SDS and nuclease-free water) 10× HEPES buffer Probe binding buffer (PBS, 0.1% (wt/vol) BSA (Thermo Scientific), 0.1 mg/ml sonicated salmon sperm DNA (Invitrogen), 6.7 nM for each isotype) CRITICAL Most solutions are made fresh with each experiment.

    Article Title: Unveiling the cut-and-repair cycle of designer nucleases in human stem and T cells via CLEAR-time dPCR
    Article Snippet: .. Droplets were generated using Automated Droplet Generation Oil for Probes (Bio-Rad) and DG32TM Automated Droplet Generator Cartridges with the automatic droplet generator (Bio-Rad QX200 AutoDG), and the plate was sealed with a PCR Plate Heat Seal (Bio-Rad) and PX1TM PCR Plate Sealer (Bio-Rad). .. PCR was performed using the C1000 TouchTM Thermal Cycler with 96-Deep Well Reaction (Bio-Rad) and droplets read using a QX200 Droplet Reader (Bio-Rad).

    Article Title: Detection of Novel Fish Viruses in Wisconsin Sportfish: Implications of Co-Infections for Disease Emergence.
    Article Snippet: We placed samples on 0.1 mL MicroAmp Optical 96- Well Reaction Plates (Thermofisher Scientific, MA, USA)/Hard- Shell 384- Well PCR Plates (skirted; Bio- Rad, CA, USA) alongside a no- template control, a negative extraction control, a gBlock standard and an RNA standard. .. We then sealed plates with PCR Plate Heat Seal (BIO- RAD, CA, USA) and prepared them on an epMotion 5075 (Eppendorf, CT, USA) liquid handling machine, after which we ran PCRs on a CFX Opus 384/96 Real- Time PCR System (Bio- Rad, CA, USA) with the following cycle settings: 45°C for 15 min, 95°C for 2 min, 40 cycles of 95°C for 15 s and 60°C for 1 min. We ran samples in duplicate and considered them positive if a sigmoidal amplification curve was present in both replicates above the fluorescence threshold. ..

    Article Title: DNA-PKcs inhibitor AZD7648 reveals sgRNA cross-contaminants and enhanced sensitivity of genome engineering off-target activity in HSPCs.
    Article Snippet: .. Droplets were generated using Automated Droplet Generation Oil for Probes (BioRad) and DG32TM Automated Droplet Generator Cartridges with the automatic droplet generator (Bio-Rad AutoDG), and the plate sealed with a PCR Plate Heat Seal (Bio-Rad) and PX1TM PCR Plate Sealer (Bio-Rad). .. PCR was performed using the C1000 TouchTM Thermal Cycler with 96-Deep Well Reaction (Bio-Rad) and droplets read using a QX600 Droplet Reader (Bio-Rad).

    Article Title: Detection of Novel Fish Viruses in Wisconsin Sportfish: Implications of Co‐Infections for Disease Emergence
    Article Snippet: We placed samples on 0.1 mL MicroAmp Optical 96‐Well Reaction Plates (Thermofisher Scientific, MA, USA)/Hard‐Shell 384‐Well PCR Plates (skirted; Bio‐Rad, CA, USA) alongside a no‐template control, a negative extraction control, a gBlock standard and an RNA standard. .. We then sealed plates with PCR Plate Heat Seal (BIO‐RAD, CA, USA) and prepared them on an epMotion 5075 (Eppendorf, CT, USA) liquid handling machine, after which we ran PCRs on a CFX Opus 384/96 Real‐Time PCR System (Bio‐Rad, CA, USA) with the following cycle settings: 45°C for 15 min, 95°C for 2 min, 40 cycles of 95°C for 15 s and 60°C for 1 min. We ran samples in duplicate and considered them positive if a sigmoidal amplification curve was present in both replicates above the fluorescence threshold. ..

    Article Title: Unveiling the cut-and-repair cycle of designer nucleases in human stem and T cells via CLEAR-time dPCR.
    Article Snippet: .. Droplets were generated using Automated Droplet Generation Oil for Probes (Bio-Rad) and DG32TM Automated Droplet Generator Cartridges with the automatic droplet generator (Bio-Rad QX200 AutoDG), and the plate was sealed with a PCR Plate Heat Seal (Bio-Rad) and PX1TM PCR Plate Sealer (Bio-Rad). .. PCR was performed using the C1000 TouchTM Thermal Cycler with 96- Deep Well Reaction (Bio-Rad) and droplets read using a QX200 Droplet Reader (Bio-Rad).

    Adhesive:

    Article Title: Proximity sequencing for the detection of mRNA, extracellular proteins and extracellular protein complexes in single cells
    Article Snippet: 5× Maxima H minus RT buffer (Thermo Scientific, cat. no. EP0752) Ficoll Solution (20%; Sigma-Aldrich, cat. no. F5415–25 ml) Exonuclease I and 1× exonuclease I buffer ((New England Biolabs, cat. no. M0293) Nextera XT DNA library preparation kit (Illumina, cat. no. FC-131–1024) Chromium Next GEM Chip G single cell kit (10× Genomics, cat. no. 1000127) Chromium Next GEM single cell 3′ GEM, library and gel bead kit v.3.1 (10× Genomics, cat. no. 1000268) Dual index kit TT set A, 96 reactions (10× Genomics, cat. no. 1000215) AMPure XP (Beckman Coulter, cat. no. A63881) Buffer EB (Qiagen, cat. no. 19086) Agilent high-sensitivity D5000 ScreenTape (Agilent, cat. no. 5067–5592) Agilent high-sensitivity D5000 reagents (Agilent, cat. no. 5067–5593) Agilent HS NGS fragment kit (1–6,000 bp), 500 (Agilent, cat. no. DNF-474–0500) Qubit 1× double-stranded DNA high-sensitivity assay kit (Invitrogen, cat. no. Q33231 ) Qubit assay tubes (Invitrogen, cat. no. Q32856 ) KAPA HiFi HotStart ReadyMix (2×; KAPA Biosystems, cat. no. KK2601) SPRIselect beads (Beckman Coulter, cat. no. B23317 ) NextSeq PhiX control kit (Illumina, cat. no. FC-110–3002) NextSeq 500/550 mid-output kit v2.5 (150 cycles; Illumina, cat. no. 20024904) NextSeq 500/550 high-output kit (150 cycles; Illumina, cat. no. 20024907) 10% Sarkosyl Solution (1,000 ml; Teknova, cat. no. S3377) dl -Dithiothreitol solution (DTT, 1 M; Sigma-Aldrich, cat. no. 43816–10 ml) .. Microcentrifuge tube, 1.5 ml DNA LoBind tube, 1.5 ml (Eppendorf, cat. no. 022431021) Falcon polystyrene conical tube, 15 and 50 ml Filter tips (P1000, P200, P100 and P10) Rainin tips (P20 and P200) 96-well plate (Eppendorf, Bio-Rad) Hemocytometer (Hausser Scientific, cat. no. 3520) Falcon cell strainers, 40 μm (Fisher Scientific, cat. no. 08–771-1) Magnetic disc (V&P Scientific, cat. no. 772DP-N42–5-2) Plastic tubes (Scientific Commodities, cat. no. BB31695PE/2) Magnetic stirrer (V&P Scientific, cat. no. 710D2) DiaMag 0.2-ml magnetic rack (Diagenode, cat. no. B04000001) DynaMag-96 side magnet (Invitrogen, cat. no. 12331D) Themal cycler (Bio-Rad C1000 Touch, cat. no. 1851196) PX1 PCR plate sealer (Bio-Rad, cat. no. 1814000) PCR plate heat seal, foil, pierceable (Bio-Rad, cat. no. 1814040) Adhesive PCR plate foils (Thermo Scientific, cat. no. AB-0626) Nanodrop UV–visible spectrophotometer (Thermo Scientific, cat. no. ND-ONE-W) Qubit 4.0 fluorometer (Invitrogen, cat. no. Q33238 ) Agilent 5200 fragment analyzer (Agilent) Agilent 4200 TapeStation (Agilent) Fortessa 4–15 (BD Biosciences) Cell sorter (BD FACSAria II) Pump 11 Pico Plus Elite infusion/withdrawal programmable dual syringe pump (Harvard Apparatus, cat. no. 70–4506) Chromium Controller (10× Genomics, cat. no. 1000204) NextSeq 500/550 (Illumina) .. TE-TW (10 mM Tris pH 8.0, 1 mM EDTA, 0.01% (wt/vol) Tween-20 and nuclease-free water) TE-SDS (10 mM Tris pH 8.0, 1 mM EDTA, 0.5% (wt/vol) SDS and nuclease-free water) 10× HEPES buffer Probe binding buffer (PBS, 0.1% (wt/vol) BSA (Thermo Scientific), 0.1 mg/ml sonicated salmon sperm DNA (Invitrogen), 6.7 nM for each isotype) CRITICAL Most solutions are made fresh with each experiment.

    Spectrophotometry:

    Article Title: Proximity sequencing for the detection of mRNA, extracellular proteins and extracellular protein complexes in single cells
    Article Snippet: 5× Maxima H minus RT buffer (Thermo Scientific, cat. no. EP0752) Ficoll Solution (20%; Sigma-Aldrich, cat. no. F5415–25 ml) Exonuclease I and 1× exonuclease I buffer ((New England Biolabs, cat. no. M0293) Nextera XT DNA library preparation kit (Illumina, cat. no. FC-131–1024) Chromium Next GEM Chip G single cell kit (10× Genomics, cat. no. 1000127) Chromium Next GEM single cell 3′ GEM, library and gel bead kit v.3.1 (10× Genomics, cat. no. 1000268) Dual index kit TT set A, 96 reactions (10× Genomics, cat. no. 1000215) AMPure XP (Beckman Coulter, cat. no. A63881) Buffer EB (Qiagen, cat. no. 19086) Agilent high-sensitivity D5000 ScreenTape (Agilent, cat. no. 5067–5592) Agilent high-sensitivity D5000 reagents (Agilent, cat. no. 5067–5593) Agilent HS NGS fragment kit (1–6,000 bp), 500 (Agilent, cat. no. DNF-474–0500) Qubit 1× double-stranded DNA high-sensitivity assay kit (Invitrogen, cat. no. Q33231 ) Qubit assay tubes (Invitrogen, cat. no. Q32856 ) KAPA HiFi HotStart ReadyMix (2×; KAPA Biosystems, cat. no. KK2601) SPRIselect beads (Beckman Coulter, cat. no. B23317 ) NextSeq PhiX control kit (Illumina, cat. no. FC-110–3002) NextSeq 500/550 mid-output kit v2.5 (150 cycles; Illumina, cat. no. 20024904) NextSeq 500/550 high-output kit (150 cycles; Illumina, cat. no. 20024907) 10% Sarkosyl Solution (1,000 ml; Teknova, cat. no. S3377) dl -Dithiothreitol solution (DTT, 1 M; Sigma-Aldrich, cat. no. 43816–10 ml) .. Microcentrifuge tube, 1.5 ml DNA LoBind tube, 1.5 ml (Eppendorf, cat. no. 022431021) Falcon polystyrene conical tube, 15 and 50 ml Filter tips (P1000, P200, P100 and P10) Rainin tips (P20 and P200) 96-well plate (Eppendorf, Bio-Rad) Hemocytometer (Hausser Scientific, cat. no. 3520) Falcon cell strainers, 40 μm (Fisher Scientific, cat. no. 08–771-1) Magnetic disc (V&P Scientific, cat. no. 772DP-N42–5-2) Plastic tubes (Scientific Commodities, cat. no. BB31695PE/2) Magnetic stirrer (V&P Scientific, cat. no. 710D2) DiaMag 0.2-ml magnetic rack (Diagenode, cat. no. B04000001) DynaMag-96 side magnet (Invitrogen, cat. no. 12331D) Themal cycler (Bio-Rad C1000 Touch, cat. no. 1851196) PX1 PCR plate sealer (Bio-Rad, cat. no. 1814000) PCR plate heat seal, foil, pierceable (Bio-Rad, cat. no. 1814040) Adhesive PCR plate foils (Thermo Scientific, cat. no. AB-0626) Nanodrop UV–visible spectrophotometer (Thermo Scientific, cat. no. ND-ONE-W) Qubit 4.0 fluorometer (Invitrogen, cat. no. Q33238 ) Agilent 5200 fragment analyzer (Agilent) Agilent 4200 TapeStation (Agilent) Fortessa 4–15 (BD Biosciences) Cell sorter (BD FACSAria II) Pump 11 Pico Plus Elite infusion/withdrawal programmable dual syringe pump (Harvard Apparatus, cat. no. 70–4506) Chromium Controller (10× Genomics, cat. no. 1000204) NextSeq 500/550 (Illumina) .. TE-TW (10 mM Tris pH 8.0, 1 mM EDTA, 0.01% (wt/vol) Tween-20 and nuclease-free water) TE-SDS (10 mM Tris pH 8.0, 1 mM EDTA, 0.5% (wt/vol) SDS and nuclease-free water) 10× HEPES buffer Probe binding buffer (PBS, 0.1% (wt/vol) BSA (Thermo Scientific), 0.1 mg/ml sonicated salmon sperm DNA (Invitrogen), 6.7 nM for each isotype) CRITICAL Most solutions are made fresh with each experiment.

    Real-time Polymerase Chain Reaction:

    Article Title: Detection of Novel Fish Viruses in Wisconsin Sportfish: Implications of Co-Infections for Disease Emergence.
    Article Snippet: We placed samples on 0.1 mL MicroAmp Optical 96- Well Reaction Plates (Thermofisher Scientific, MA, USA)/Hard- Shell 384- Well PCR Plates (skirted; Bio- Rad, CA, USA) alongside a no- template control, a negative extraction control, a gBlock standard and an RNA standard. .. We then sealed plates with PCR Plate Heat Seal (BIO- RAD, CA, USA) and prepared them on an epMotion 5075 (Eppendorf, CT, USA) liquid handling machine, after which we ran PCRs on a CFX Opus 384/96 Real- Time PCR System (Bio- Rad, CA, USA) with the following cycle settings: 45°C for 15 min, 95°C for 2 min, 40 cycles of 95°C for 15 s and 60°C for 1 min. We ran samples in duplicate and considered them positive if a sigmoidal amplification curve was present in both replicates above the fluorescence threshold. ..

    Article Title: Detection of Novel Fish Viruses in Wisconsin Sportfish: Implications of Co‐Infections for Disease Emergence
    Article Snippet: We placed samples on 0.1 mL MicroAmp Optical 96‐Well Reaction Plates (Thermofisher Scientific, MA, USA)/Hard‐Shell 384‐Well PCR Plates (skirted; Bio‐Rad, CA, USA) alongside a no‐template control, a negative extraction control, a gBlock standard and an RNA standard. .. We then sealed plates with PCR Plate Heat Seal (BIO‐RAD, CA, USA) and prepared them on an epMotion 5075 (Eppendorf, CT, USA) liquid handling machine, after which we ran PCRs on a CFX Opus 384/96 Real‐Time PCR System (Bio‐Rad, CA, USA) with the following cycle settings: 45°C for 15 min, 95°C for 2 min, 40 cycles of 95°C for 15 s and 60°C for 1 min. We ran samples in duplicate and considered them positive if a sigmoidal amplification curve was present in both replicates above the fluorescence threshold. ..

    Amplification:

    Article Title: Detection of Novel Fish Viruses in Wisconsin Sportfish: Implications of Co-Infections for Disease Emergence.
    Article Snippet: We placed samples on 0.1 mL MicroAmp Optical 96- Well Reaction Plates (Thermofisher Scientific, MA, USA)/Hard- Shell 384- Well PCR Plates (skirted; Bio- Rad, CA, USA) alongside a no- template control, a negative extraction control, a gBlock standard and an RNA standard. .. We then sealed plates with PCR Plate Heat Seal (BIO- RAD, CA, USA) and prepared them on an epMotion 5075 (Eppendorf, CT, USA) liquid handling machine, after which we ran PCRs on a CFX Opus 384/96 Real- Time PCR System (Bio- Rad, CA, USA) with the following cycle settings: 45°C for 15 min, 95°C for 2 min, 40 cycles of 95°C for 15 s and 60°C for 1 min. We ran samples in duplicate and considered them positive if a sigmoidal amplification curve was present in both replicates above the fluorescence threshold. ..

    Article Title: Detection of Novel Fish Viruses in Wisconsin Sportfish: Implications of Co‐Infections for Disease Emergence
    Article Snippet: We placed samples on 0.1 mL MicroAmp Optical 96‐Well Reaction Plates (Thermofisher Scientific, MA, USA)/Hard‐Shell 384‐Well PCR Plates (skirted; Bio‐Rad, CA, USA) alongside a no‐template control, a negative extraction control, a gBlock standard and an RNA standard. .. We then sealed plates with PCR Plate Heat Seal (BIO‐RAD, CA, USA) and prepared them on an epMotion 5075 (Eppendorf, CT, USA) liquid handling machine, after which we ran PCRs on a CFX Opus 384/96 Real‐Time PCR System (Bio‐Rad, CA, USA) with the following cycle settings: 45°C for 15 min, 95°C for 2 min, 40 cycles of 95°C for 15 s and 60°C for 1 min. We ran samples in duplicate and considered them positive if a sigmoidal amplification curve was present in both replicates above the fluorescence threshold. ..

    Fluorescence:

    Article Title: Detection of Novel Fish Viruses in Wisconsin Sportfish: Implications of Co-Infections for Disease Emergence.
    Article Snippet: We placed samples on 0.1 mL MicroAmp Optical 96- Well Reaction Plates (Thermofisher Scientific, MA, USA)/Hard- Shell 384- Well PCR Plates (skirted; Bio- Rad, CA, USA) alongside a no- template control, a negative extraction control, a gBlock standard and an RNA standard. .. We then sealed plates with PCR Plate Heat Seal (BIO- RAD, CA, USA) and prepared them on an epMotion 5075 (Eppendorf, CT, USA) liquid handling machine, after which we ran PCRs on a CFX Opus 384/96 Real- Time PCR System (Bio- Rad, CA, USA) with the following cycle settings: 45°C for 15 min, 95°C for 2 min, 40 cycles of 95°C for 15 s and 60°C for 1 min. We ran samples in duplicate and considered them positive if a sigmoidal amplification curve was present in both replicates above the fluorescence threshold. ..

    Article Title: Detection of Novel Fish Viruses in Wisconsin Sportfish: Implications of Co‐Infections for Disease Emergence
    Article Snippet: We placed samples on 0.1 mL MicroAmp Optical 96‐Well Reaction Plates (Thermofisher Scientific, MA, USA)/Hard‐Shell 384‐Well PCR Plates (skirted; Bio‐Rad, CA, USA) alongside a no‐template control, a negative extraction control, a gBlock standard and an RNA standard. .. We then sealed plates with PCR Plate Heat Seal (BIO‐RAD, CA, USA) and prepared them on an epMotion 5075 (Eppendorf, CT, USA) liquid handling machine, after which we ran PCRs on a CFX Opus 384/96 Real‐Time PCR System (Bio‐Rad, CA, USA) with the following cycle settings: 45°C for 15 min, 95°C for 2 min, 40 cycles of 95°C for 15 s and 60°C for 1 min. We ran samples in duplicate and considered them positive if a sigmoidal amplification curve was present in both replicates above the fluorescence threshold. ..



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